FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865017
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34000.18230396827482004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20600.036.1838382
GTGGTAT21150.036.1784481
TAGACGC601.7205439E-632.0771876
CCTAGCG453.8001133E-430.5505187
TCGTCGT400.0078734927.5006312
CGAATTA3300.026.6672815
CTTACGC651.0889511E-425.3858783
CATGCGG651.0895466E-425.38383716
CGACGAG550.001227060924.99923124
GCCCCGA3650.024.11014211
GTATCAA67750.023.8069531
CCGAATT3750.023.46720314
CCCCGAA4050.023.0869512
CAATCGC1605.638867E-1122.34306339
GGTATCA38150.022.2214151
GACAGGT5800.021.8295781
CGACGCC1406.4774213E-921.60590231
AGCCCCG4150.021.20587510
AGAGTAC40600.021.1334911
TATCAAC75450.021.0708072
Produced by FastQC (version 0.11.5)