FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751507
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.13999373111269323No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.13422726828953582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17300.040.2900852
GTGGTAT18200.037.365761
GTACTAG1850.034.462341
GGTATCA26550.033.2984541
GTATCAA43900.029.820261
TATCAAC47350.029.7103982
AGCGTCA3150.028.3340873
GTCGAGG8650.027.5120378
GCGTCAG3300.027.0461734
CAACGCA52050.027.0276135
ATCAACG52650.026.5581593
TCAACGC54650.025.7417624
AACGCAG55200.025.7162636
GGCGTCG1001.056859E-425.4977658
GCCGAAC601.2771947E-524.79161864-65
CGTCAGA3800.024.6059155
AGTCGAG12200.024.0347777
TCGAGGG1251.7526214E-523.7979139
TCGAGGT8850.023.529019
CGCAGAG60500.023.320278
Produced by FastQC (version 0.11.5)