FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777177
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.12975634953637144No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.1289685833206259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17200.034.1050571
GTATCAA46800.033.1524161
AGCGTCA2600.032.697353
GGTATCA28800.031.8808171
TGGTATC20050.030.9532782
TATCAAC56600.029.2897952
CAACGCA56550.029.23975
ATCAACG57100.028.9580573
GCGTCAG3100.028.7947644
GTCGAGG9400.028.483638
TCAACGC58850.028.169174
AACGCAG59300.028.098776
ATACGGG2600.027.793531
CGTACAC1255.695674E-727.2041973
GCCGACC408.6157426E-426.56211574-75
CGCAGAG63950.025.7189378
TCGAGGT10000.025.498919
ACGCAGA67400.024.5916297
AGTCGAG12350.024.4328427
GCAGAGT70250.023.5328049
Produced by FastQC (version 0.11.5)