FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777177
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39610.22288157004057557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGG303.493751E-545.82941424
CGACGAG558.709303E-734.9970124
ACCGTCG401.9029173E-434.37206323
GTGGTAT14650.033.2542421
TGGTATC16300.029.357452
CTACCGG400.00788296527.4937823
GACGGTC550.001225819225.00349448
GACCGTC550.001227026924.99927122
GTATCAA50300.024.7332861
GGTATCA26300.023.2331771
ATGCGAC852.5713629E-522.64703421
TATACGG1003.6153942E-622.0012152
TGCGACG1003.6180445E-621.99935722
TATCAAC56350.021.9133662
AGAGTAC30450.021.67485411
ATCAACG56850.021.4727173
CTGGTCG903.994635E-521.39067550
GGCGTCG650.003242572821.1514438
CAACGCA58000.020.9018125
TCTGTCG2250.020.775428
Produced by FastQC (version 0.11.5)