FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014095
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.15585163559812223No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.1345517465660756No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.10605259434137915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19400.034.8334731
AGCGTCA4700.034.3626863
TGGTATC20450.033.0441322
GTATCAA56000.032.710761
GCGTCAG5300.030.4733264
ATCAACG65000.030.0123983
CAACGCA65800.029.6482415
GGTATCA34000.029.6258491
TATCAAC67150.029.430492
TCAACGC66750.029.2262824
AACGCAG69750.028.030176
AAGTACG4750.027.7376351
ACGGGCG1255.701331E-727.2014545
GTATACG1255.702568E-727.2007791
CGTCAGA6550.025.9555855
GCAGCGT6250.025.840741
GTCGAGG12400.025.7063838
GCGCGTA759.833275E-825.49756612-13
TGCGCGT759.833275E-825.49756612-13
CGCAGAG77550.025.0458498
Produced by FastQC (version 0.11.5)