FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014095
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51760.256988870932106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTC1050.036.66433326
GTGGTAT20650.032.6526151
CGTCCGT350.004102634731.4250110
TGGTATC22200.029.6113242
CGATCAC507.0271967E-427.504436
CCTCGCG400.00787696327.498259
CGACGAG1500.027.49824724
GCGACGA1355.456968E-1226.47979523
CGAGTCC1500.025.66503127
GTATCAA60250.023.8443451
TGCGACG1502.3646862E-1123.83240922
TAGGTCG1553.6379788E-1123.06476821
CGCGGTA600.002035047622.91862343
CGCGCTC600.002035047622.91862343
AGAGTAC33800.022.85983511
ATCGTCA1950.022.56154410
GACGAGT1801.0913936E-1121.38752625
GTCGGTA903.9995095E-521.38752626
TCGTAGA650.003237116621.1577552
AGCCGCG650.003240382521.15407419
Produced by FastQC (version 0.11.5)