FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006821
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32490.16189784739147137No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.16040294575350766No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.10618784635002324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21650.043.5849152
GTGGTAT23300.040.134581
GTATCAA62450.037.5715031
AGCGTCA2950.033.1379243
TATCAAC78400.032.7462842
CAACGCA78950.032.139695
ATCAACG80750.031.3179933
TCAACGC81450.031.2053894
GGTATCA35050.030.1969641
AACGCAG89850.028.6191526
ATACGGG3800.027.9645841
CGCAGAG93750.027.1531938
ACGCAGA100950.025.427057
GTCGAGG12450.024.9183278
GCAGAGT102350.024.9125379
AGTCGAG17650.023.8372347
TCGAGGT12800.022.5763389
GACAGGT8350.022.398461
GCGTCAG4400.022.217474
CAGCGTC4550.021.4860972
Produced by FastQC (version 0.11.5)