FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619846
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.2661364104982819No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29850.18427677692817712No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27090.16723812016697884No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT23360.14421123983391015No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.1435321629340073No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.11297370243837995No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT16730.10328142304885773No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16520.10198500351267961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22100.058.6562542
GTGGTAT21800.056.349271
AAGTACG6700.046.9465751
GTACGAC1400.039.4659043
GGTATCA40600.037.3756871
GTATCAA77100.034.236091
ATACGGG4150.032.7754671
TATCAAC101000.032.5706442
ATATCAA21600.031.2889021
ATCAACG108350.030.3219643
GTCGAGG17800.030.3205348
TCAACGC109500.030.0423284
CAACGCA110700.029.7934555
AACGCAG116400.028.3344966
TACGACC1606.2482286E-926.5635914
TCGAGGT18950.026.4630159
AGTCGAG23950.026.2608817
GGGTACG1159.167932E-625.8730241
GGTACGA1351.1157863E-625.1862162
CGCAGAG136450.024.1368878
Produced by FastQC (version 0.11.5)