FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619846
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78420.4841200953670905No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27400.16915188233943226No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17110.10562732506670387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCG150.005784563354.99873451
CGTAACG305.4345037E-754.9936423
GTGGTAT26450.042.249891
TGGTATC26200.042.1108322
GTACGCA403.937319E-641.25542411
GCGACCG403.9431743E-641.24650218
CGACCGT458.851575E-636.66355519
GCTATCG350.00409872831.43076136
GTGTCGA453.7946776E-430.55768255
AGCGACC553.4937373E-529.99745617
ACCGGTG400.00787257927.50106645
GCGCGTT400.00787495227.49936751
CGACGCT400.00787851227.4968228
TCGTACC651.08552296E-425.3973642
GGTATCA48450.024.2582421
GTATCAA91500.024.095541
CGGACGT1203.0009687E-822.91755546
CTACGAC903.9909766E-521.393023
CCGGTCG1559.0039975E-1021.2931219
GACAGGT3250.021.1729741
Produced by FastQC (version 0.11.5)