FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215524
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18260.8472374306341753No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4400.20415359774317476No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2250.10439672611866893No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG150.005768578555.02089735
ATGGCGA150.005779187754.99535818
CGATTTC150.005779187754.99535820
TAGCCCC150.005779187754.99535825
TCGTGCT150.005779187754.99535829
GGAACGA150.005779187754.99535825
TCGCCAC257.8454876E-443.9962921
GTGGTAT2800.041.2752571
TGGTATC2950.039.1583252
TCTAGAA401.8973768E-434.372153
GTATCAA14500.032.071351
CGAGGAA350.004094001431.42591929
TGCAGGT350.004094001431.42591945
CGCCACC350.004094001431.42591922
TACGGGT453.7872855E-430.55297515
TACGGGC553.479834E-529.99746915
AGAGTAC10300.028.5655511
CAGAGTA10900.027.74995210
GACTTGC400.00786241627.49767919
CGAATGT400.00786241627.49767928
Produced by FastQC (version 0.11.5)