FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246775
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.19815621517576737No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.14588187620301893No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.10819572485057237No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAT200.003218960763.75767
AGCGTCA354.8215585E-448.5673753
GGTATCA5550.039.8164981
CTATGCA450.001659543737.782289
ACTAGAG450.001659543737.782285
GTATCAA9150.035.2976041
GCGTCAG500.002783136434.004054
TATCAAC11400.032.8042832
GTATTAC652.5488253E-432.689581
GTGGTAT2550.031.6640221
TACACTA550.004438717430.9127775
TCAACGC12400.029.8221024
CAACGCA12600.029.3487365
TGGTATC2900.029.3078962
ATCAACG12650.029.226813
GTCGAGG1751.8189894E-1129.1463328
TCGAGGT1951.8189894E-1228.336719
TACTACA1054.508469E-628.330972
GTAGGAC600.006798153728.3309693
AACGCAG13750.026.8941156
Produced by FastQC (version 0.11.5)