FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246775
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9580.38820788167358933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGA150.00577293655.0127836
CGATAAC202.6092128E-455.0016259
CCCTAGT257.819828E-444.028081
ACGCGAG257.835472E-444.0102237
TAACGTT257.8433036E-444.001312
GTCGTTA257.8433036E-444.001328
TTAAACG358.646419E-539.28687322
GTGGTAT2450.038.187621
TGGTATC2400.037.798292
TCGTTAC300.001922416736.66774729
TGGATCC458.814257E-636.66774733
ATAACGT300.001922416736.66774711
GGTCGTT350.004092857731.42949927
CGTTACC350.004092857731.42949930
GACGATA350.004096922531.4231267
GTATCAA12600.030.5750561
TATCAAC13700.028.292282
GTATTAC400.00783690527.517551
GGATCCA506.998333E-427.51196734
CGCGAGT400.00785243927.5063938
Produced by FastQC (version 0.11.5)