FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248666
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12340.49624797921710245No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC150.00577871554.99899741
CGTCCCC150.00577871554.99899755
GTCAGCG257.845269E-443.99919543
TAGTAAC257.845269E-443.9991957
CGTTTAG403.9224506E-641.25754538
CATTGCG300.001922896136.66599729
GTCGTCT300.001922896136.66599753
TGGTATC2800.036.3386232
ACGGGAT401.8974756E-434.3743716
GTGGTAT2950.033.5722161
GTATATG501.8095237E-533.012681
AGAGTAC7750.031.934911
TAGGTCG350.00409387531.42799821
TCGTCTA350.00409387531.42799854
GTTATAG350.00409387531.4279983
GTATCAA13550.030.6575341
GGTCGTC553.4803936E-529.99944952
TTTCGTC553.4803936E-529.99944929
CGTCTGC553.4803936E-529.99944932
TCGTCTG553.4803936E-529.99944931
Produced by FastQC (version 0.11.5)