FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168743
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.14756167663251216No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.129783161375583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG1050.048.5780378
GTATCAA4200.046.553951
TCGAGGT1153.6379788E-1240.6456539
TATCAAC5600.040.2286872
ATCAACG5700.038.7772033
TCAACGC5700.038.7772034
AGTCGAG1550.038.392327
CAACGCA5850.037.7829175
GGTATCA2150.035.586231
AACGCAG6350.035.4772646
AAGTACG500.00278042234.0046271
TCTTAGG500.00278042234.0046272
AGAGTCG3500.032.7901735
GTCTTAG652.542146E-432.6967541
GACAGGT1051.226017E-732.3853571
CATACAC703.925995E-430.3612733
GAGTCGA3850.029.809256
ACGCAGA7850.028.6981727
TCGCACC300.006794356728.33718750-51
TCGACGT755.8901304E-428.328799
Produced by FastQC (version 0.11.5)