FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168743
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5500.3259394463770349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT202.6040297E-455.01363455
CGCCTGA150.00577620154.99733433
CGGGAAA753.6379788E-1240.33137517
TGGTATC1750.036.1411062
GTGGTAT1850.034.1976661
GTATGAA501.8077795E-533.0081831
GATAAGC350.00409078631.42704812
TACTCAC350.00409078631.4270485
AGAGTAC4100.029.51076511
GTATCAA6800.028.7203541
TAGACTG507.0088974E-427.4986655
TACGGGC400.00785628327.49866515
AGTACGG4450.027.18969313
CAGAGTA4400.026.87369510
TATCAAC6950.026.5095062
ACGGGAA1156.530172E-1026.30307216
ACGGGGG1057.1704562E-926.18920516
ATAACAT550.001222565824.9987873
AACGCAG7500.024.9321236
ATCAACG7300.024.8618073
Produced by FastQC (version 0.11.5)