FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328010
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10740.32742904179750615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCCG257.857463E-443.9895065
GGCACGC300.001922989336.6691137
TGGTATC2400.033.231382
GTGGTAT2650.032.1915971
GTACGGA453.791595E-430.5529273
CGCAGAT400.00786836527.49763550
GTATCAA10550.026.8665241
TATCAAC11250.025.6683752
AACGCAG12650.023.6900026
CAACGCA12100.023.6307265
TAATACT701.7997104E-423.565814
TCAACGC12150.023.5334824
ATCAACG12300.023.2500323
AAACGCA600.002035266722.9112035
AGAGTAC6900.022.32032811
GGTATCA4750.022.0148981
CGCTGCC1757.2759576E-1222.00146555
CGAGGGT903.9776998E-521.39357810
TCCGTCA650.003230763621.15848410
TAGACCA650.00323935121.1488025
Produced by FastQC (version 0.11.5)