FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411145
Sequences flagged as poor quality0
Sequence length61
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36360.8843595325250216No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA16710.4064259567792385No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA15130.3679966921645648No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC12840.31229858079266437No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12810.3115689112113731No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9570.23276459643191577No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8460.20576682192413867No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA7770.18898442155443942No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTT7460.18144450254776295No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7290.1773097082537791No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7110.17293169076603146No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6500.15809507594644226No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5660.13766432767028663No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA5650.1374211044765229No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC5590.13596176531394033No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA5580.1357185421201766No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4870.1184496953629498No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4350.10580208928723442No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA4220.1026401877683056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGC150.00577996455.00243417
TGACTTT150.00577996455.00243439
GGTCGGT150.005782748654.9957425
GTCGGTA150.005782748654.9957426
CGACTAA202.614218E-454.98904428
ATGTCAT150.0057883254.9823576
TGTGTAC150.0057883254.9823576
CGTGGGC257.844154E-444.00731
TACTGAG257.844154E-444.00732
TGCTTAA2000.044.00194519
CATGCGA257.8488584E-444.00194511
ATGCGAC257.8488584E-444.00194512
CGAACCG257.853565E-443.9965923
ATCCGTC257.853565E-443.9965926
CCGAACC257.853565E-443.9965922
TCGGAGA257.858274E-443.9912388
GTCGTAC257.858274E-443.99123830
CGTACCA257.858274E-443.99123832
CGTCGTA257.858274E-443.99123829
CTTAAAC1850.043.11001621
Produced by FastQC (version 0.11.5)