FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452108
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.1593545383676696No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.11755323984166466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12750.040.005061
GTATCAA45150.036.150751
TATCAAC50850.034.3518262
AGCGTCA3650.032.6034933
ATCAACG56000.031.1926843
CAACGCA56700.030.8814815
GGTATCA26050.030.6756611
TCAACGC57950.030.4353734
TGGTATC17600.029.9438722
AACGCAG60850.028.9848826
CGTCAGA4300.028.6624645
CGCAGAG65300.026.6833118
GCGTCAG4550.026.153554
ACGCAGA67000.026.1331337
GCAGAGT70850.024.7130559
AAGTACG4000.023.3779541
GTCGAGG10050.022.8347748
CGGGAAC3000.022.666414
TCGAGGT10150.021.35379
CAGAGTA41700.021.045210-11
Produced by FastQC (version 0.11.5)