FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684994
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.2845280396616613No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.23752032864521438No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.15897949471090259No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT10240.149490360499508No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.1083221166900732No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.10452646300551538No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.10204468944253528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1300.042.498323
GTGGTAT9950.041.0034031
TGGTATC10550.040.6855932
GACAGGT2350.037.9772221
GTATCAA34550.037.639611
TATCAAC42050.035.3731582
CCATGCG500.002789741833.9961749
ATCAACG44700.033.1810153
CAACGCA45850.032.4414675
TCAACGC46150.032.1384934
GCGTCAG1750.031.570184
AACGCAG47150.031.3667356
GTCGAGG8400.030.3537278
CGTCAGA1851.8189894E-1229.8636845
AAGTACG3200.029.2175961
GGTATCA17900.029.2027571
ATACGGG1751.8189894E-1129.1417051
AGAGCGT300.006807344528.33221622-23
CGCAGAG51900.028.2482668
TCGAGGT8450.028.162519
Produced by FastQC (version 0.11.5)