FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684994
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37380.5456982104952744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG300.00192451536.66885854
GACAGGT2200.033.7865641
GTGGTAT12350.031.87661
GGTTCGC350.0041002231.42585825
TGGTATC12850.030.6138862
GTTCGCC453.7958781E-430.5529226
TCGCCCA553.4891193E-529.99960112
GTCGCCC606.314664E-527.49963611
CCACACG805.1748793E-727.49963412
GTATCAA43550.025.8546141
TGGCGAT550.001226142924.99966816
TTAAACG1151.9090294E-823.91097822
ACGGCTC701.7997506E-423.57111416
TAAGCCG701.7997506E-423.57111436
AGAGTAC27050.023.1789911
CCTATAC952.3081102E-623.1643543
TGCCGAC2150.023.02463255
TACGAAC600.00203398722.91803644
CTGGCGA600.002035715422.9146915
GCCGTCA852.568623E-522.64675939
Produced by FastQC (version 0.11.5)