FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868329
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15540.17896442477448063No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13150.15144029509552256No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT10850.12495263891911937No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG250.007793379551.0036965
GTGGTAT8250.050.4943281
GTATCAA37850.044.3610341
TGGTATC9500.044.2926832
TATCAAC45050.040.1915972
CAACGCA49100.036.7032745
ATACGGG1550.035.651851
AACGCAG51700.034.939676
AAGTACG2250.034.0063821
GTGTACG500.002786047534.0063821
ATCAACG53550.033.8913463
TCAACGC53600.033.859734
GGTATCA16450.033.8513371
GTCTATA802.3035951E-531.8809831
CGCAGAG57650.031.2544568
ACGCAGA58400.030.8566257
GCAGAGT60000.030.1011499
AGCGTCA1601.9463187E-1029.220873
GACAGGT3750.028.3386521
GCGTCAG1807.6761353E-1025.9741044
Produced by FastQC (version 0.11.5)