FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868329
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40910.47113478877245835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10500.037.7480161
TGGTATC10350.037.7349242
TAGACCG350.00410659531.4168245
GTATCAA42750.027.8143271
CGCTAAG400.00786432627.5055635
GGTACGA400.00786653827.5039772
CGCCGGG400.0078753927.49763732
TACACGT400.00788646727.489725
GCGGTTT651.0871678E-425.3897540
TATCAAC48350.024.801932
AGAGTAC34550.024.35391411
GGTATCA20350.024.2108231
CAGAGTA35700.023.80184710
CGTCTTA600.00203614722.914719
CAACGCA52850.022.6783685
ATCAACG54450.022.1698133
TCAACGC54950.022.0155894
TTAAACG752.8735425E-421.9981122
AACGCAG55050.021.97186
TCGAGGG2900.021.8109849
Produced by FastQC (version 0.11.5)