FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45033
Sequences flagged as poor quality0
Sequence length91
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7281.6165922767748098No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT6861.523327337730109No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.9815024537561344No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.9726200786090201No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.850487420336198No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.7150311993427042No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.6595163546732397No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.6173250727244465No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.615104478937668No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.6017809162169964No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.4907512268780672No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.47742766415739574No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.3997068816201452No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.3464126307374592No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.3441920369506806No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.32420669286967335No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.3108831301490019No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC1330.29533897364155176No Hit
ATGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.28867719228121597No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.27757422334732307No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.26869184820020875No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.25758887926631585No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.2420447227588657No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.2287211600381942No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT830.18430928430262253No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.17542690915550818No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG770.17098572158195102No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.16654453400839384No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA740.16432394022161526No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.16210334643483668No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA680.15100037750094375No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA670.14877978371416517No Hit
TATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.1421180023538294No Hit
GTGGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA640.1421180023538294No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA630.13989740856705082No Hit
GTATCAACGCAGAGTACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA620.13767681478027224No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG620.13767681478027224No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT610.13545622099349366No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGG600.13323562720671508No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG600.13323562720671508No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT570.12657384584637932TruSeq Adapter, Index 1 (95% over 22bp)
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG540.11991206448604355No Hit
AAAAAAAGTCGACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCC520.1154708769124864No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.11325028312570783No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.11102968933892922No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.11102968933892922No Hit
ATGGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA500.11102968933892922No Hit
AGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA480.10658850176537206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATC150.001023153184.9988862
ATATCAA2350.074.2304461
AGTATCA750.073.747651
ATGGTAT302.5900044E-670.9111941
TGGTATC3000.066.5824662
AAGTACG900.066.1837841
ATACGGG401.899989E-763.8200761
GACAGAT200.003203550463.7491659
GTGGTAT2750.063.4332921
CAGTGGT401.4264415E-553.18341
GTATAAG551.7123639E-646.4146041
AAGAGAC551.7255697E-646.363035
AGAGACA551.7255697E-646.363036
TGCGTGA209.274209E-442.49944354-55
TATAAGA603.1406853E-642.4994432
TTCTGCG255.4122895E-542.49944352-53
TAAGAGA603.1406853E-642.4994434
CTTAAAC303.1761647E-642.49944320-21
CGTGAAA303.1761647E-642.49944356-57
TACCACT303.1761647E-642.49944312-13
Produced by FastQC (version 0.11.5)