FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326980
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113670.8566067310735657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8200.030.17672
GGTATCA20400.030.0845581
GTGGTAT8500.029.787761
GTATCAA42000.029.6181581
TATCAAC47500.026.0472552
TCAACGC48150.025.6288724
CAACGCA49350.025.0613695
ATCAACG49400.025.0397783
AACGCAG50450.024.4650696
ACGCAGA51300.024.1132877
CGCAGAG51100.024.1009838
AGAGTAC50200.023.99178511
CAGAGTA50700.023.8085210
GCAGAGT53900.022.9553139
AGTACGG56350.021.32695613
GAGTACG59550.020.22710212
ACGGGAT12000.019.939416
GTACGGG62650.019.05137614
TACGGGG24800.018.63080815
TACGGGA29200.018.5548515
Produced by FastQC (version 0.11.5)