FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069510
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53980.5047171134444746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5400.031.0872021
GTATCAA29250.031.0480021
GGTATCA12450.028.9565981
TATCAAC32250.027.7111072
CTACGCT507.042368E-427.49284
TCAACGC33650.026.2264144
ATCAACG34000.026.1990223
CAACGCA34650.025.5500585
AGAGTAC34400.025.33967411
TGGTATC6350.025.116232
CAGAGTA34950.024.9409110
AACGCAG35200.024.9165156
ACGCAGA35250.024.8811727
CGCAGAG36600.023.9634258
GCAGAGT38100.023.0953859
AGTACGG38100.022.87993413
TACGAGA903.9975967E-521.387292
TCGACAC3100.021.2887322
GAGTACG41050.021.235712
ACGGGAT7050.021.06422416
Produced by FastQC (version 0.11.5)