FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539122
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.19569598771247504No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT22320.1450177438825512No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.13020410337841964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13550.042.655132
GTGGTAT14250.040.8779451
GTATCAA46200.040.7704121
TATCAAC49900.037.1327932
ATCAACG51100.036.2596133
TCAACGC51300.036.2010884
CAACGCA51550.036.0255245
AACGCAG54100.034.484576
ACGCAGA58400.031.943397
CGCAGAG58900.031.6011078
GGTATCA24000.031.3577881
GCAGAGT67400.027.7419019
CTAACGG850.001227260324.9981963
CAGAGTA44400.024.31051610-11
AGAGTAC42450.020.77226610-11
GAGTACG52400.020.35569412-13
ACGGGAT10650.020.15047316-17
GTATATA4500.019.8422161
GTACGGG54300.019.48722614-15
ATAGTAC3359.094947E-1219.0284773
Produced by FastQC (version 0.11.5)