FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066903
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT25960.2433210891711805No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.14790472985829078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8550.045.2473451
TGGTATC8950.041.792272
GTATCAA30800.039.7520521
GGTATCA16800.036.186331
TATCAAC35300.034.9188272
ATCAACG35750.034.474443
TCAACGC35950.034.4008674
CAACGCA36550.033.719875
AACGCAG38450.032.164146
CGCAGAG40450.030.572398
ACGCAGA40350.030.5428357
GCAGAGT45050.027.450689
CAGAGTA34150.023.45716910-11
AGAGTAC32000.020.9163310-11
GAGTACG39600.020.17518812-13
CCTATAC2001.5664282E-619.1243973
GTACGGG41850.018.93818314-15
GTATTAG3900.018.5311371
AGTACGG36050.018.50756612-13
TAACCCG1200.00905327617.7077775
Produced by FastQC (version 0.11.5)