FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066903
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52130.48861049223781355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28500.029.9212321
GTGGTAT6000.029.3421151
TGGTATC5850.028.6649742
TATCAAC30800.027.4009272
TCAACGC32100.026.116284
GGTATCA13750.026.0077841
ATCAACG32500.025.796063
CAACGCA33050.025.3679645
AACGCAG33750.024.7603656
ACGCAGA33850.024.5259537
CGCAGAG34100.024.3472828
AGAGTAC33800.023.83918411
CAGAGTA34500.023.43190610
GCAGAGT37000.022.5175069
TAGTTCG752.8794166E-421.9921467
AGTACGG38200.020.80632213
CTGCCGA8700.019.91508355
GAGTACG41150.019.4474912
GTACGGG44400.017.83899514
TGCCGAC4700.017.55432955
Produced by FastQC (version 0.11.5)