FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193804
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT36440.30524273666363994No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31790.26629161905974513No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.18202317968443732No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.11492673839256695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12050.051.5042571
TGGTATC11950.051.215232
GTATCAA43050.039.2995221
TATCAAC47250.035.7102782
GGTATCA23900.033.9713481
TCAACGC50700.033.528974
ATCAACG51050.033.299093
CAACGCA51650.032.8299875
AACGCAG53450.031.7243946
ACGCAGA58200.029.1339867
CGCAGAG59500.028.4974448
GCAGAGT63000.026.9142539
CAGAGTA41250.024.00390810-11
CAGTCCG2305.4205884E-1022.1719259
AGAGTAC38800.020.59093910-11
GAGTACG49350.019.80572112-13
ACGGGAT10500.019.4268316-17
TACGCGA801.1741478E-418.59247228-29
GTATAGA2302.9046714E-718.4820211
GTACGGG52950.018.21838614-15
Produced by FastQC (version 0.11.5)