FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314853
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.15028295938785552No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT17930.13636505373604502No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.10434626532395636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9800.044.2313842
GTGGTAT10500.042.1000981
GTATCAA34800.040.7949371
TATCAAC38700.036.4572222
TCAACGC39300.035.791134
CAACGCA39850.035.190515
ATCAACG40000.035.1647833
GGTATCA19150.033.7375761
AACGCAG42950.032.650576
CGCAGAG47400.029.6726678
ACGCAGA47700.029.4860467
GCAGAGT51000.027.9113969
CAGAGTA36850.024.09992610-11
AGAGTCG15200.022.0863275
GTCGTAC1000.00315378621.2524551
AGAGTAC35000.020.4568510-11
GTATACG1050.004184170620.2404331
ACTACCG1050.004189702720.2358135
AGTCGAC16900.020.1145447
GAGTACG44350.019.44954112-13
Produced by FastQC (version 0.11.5)