FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690756
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27560.3989831431069727No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.032.4074061
GTATCAA18900.029.6960721
GTCTTAA952.4792826E-928.9605661
GTCGCTA400.007867401527.50257131
TGGTATC3200.027.4926132
TATCAAC20750.027.1613772
GTGGTAT3600.025.9840621
ATCGTAG550.0012253925.00233849
TCAACGC22400.024.9133764
CAACGCA22650.024.392125
ATCAACG22950.024.196543
AACGCAG23100.024.0376786
CGGGATA1151.9068466E-823.9135517
CGCAGAG23450.023.5633938
ACGCAGA23950.023.1845667
AGAGTAC24150.023.00088911
CAGAGTA25000.022.10886610
GCAGAGT25750.021.4633629
AGTACGG26650.020.4319513
CGATCGT700.00497136919.64469547
Produced by FastQC (version 0.11.5)