FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546663
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18950.3466486665459342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3550.032.548481
GTATCAA16450.032.1103551
TGGTATC3450.031.87082
ACGATTA350.004096934631.42987840
GCGTAAT400.00786095627.5061851
TATACTA400.00788908927.4860525
GGTATCA7500.026.7775841
TATCAAC19450.026.7112522
TCAACGC20100.025.9818364
ATCAACG20650.025.5560383
CAACGCA20550.025.4128935
AACGCAG21200.024.6359796
TGCCGAC2350.024.5822455
CGCAGAG21200.024.5063158
ACGCAGA21600.024.0524947
CTATTGT701.8051395E-423.5594714
AGAGTAC22050.023.44554311
CAGAGTA22700.023.01226210
GCAGAGT23650.021.9696149
AGTACGG24100.021.5692613
Produced by FastQC (version 0.11.5)