FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438870
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46240.3213632920277718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7850.030.8478581
GTATCAA41950.030.2399431
TGGTATC8200.029.507572
GGTATCA19150.029.4575821
TATCAAC47800.026.74792
TCAACGC49800.025.3344884
CAACGCA50400.025.1410855
ATCAACG50200.025.0822223
TGCCGAC5000.024.75644955
AACGCAG51500.024.711696
ACGCAGA53000.023.9612757
CGCAGAG53200.023.8720268
AGAGTAC51750.023.5349211
CAGAGTA52650.023.28926810
GCAGAGT58350.021.9094479
AGTACGG58200.020.65041413
GTATTAC1802.6739144E-1019.8738881
GAGTACG61850.019.69444512
TACCGAC700.00498816119.6358
GTATAGG2250.019.5681361
Produced by FastQC (version 0.11.5)