FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084072
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.1627198193477924No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT15390.1419647403493495No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12850.11853456227999615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7900.050.028522
GTGGTAT8950.045.117241
GTATCAA36700.042.1577341
GGTATCA19200.039.8486141
TATCAAC41050.037.8904462
TCAACGC42750.036.1832054
ATCAACG43600.035.5752683
CAACGCA43550.035.518535
AACGCAG45750.033.8105356
ACGCAGA48000.032.31427
CGCAGAG48350.032.0802848
GCAGAGT54250.028.8263669
CAGAGTA40300.024.5165710-11
TAGGACG950.002344498922.3659934
GAGTACG44950.021.88583412-13
AGAGTAC38700.020.47904410-11
GTTAGCC2101.118151E-720.2358993
GTACGGG48700.020.0696914-15
GTCCATA2853.7289283E-1019.3883321
GTATTAT2302.906545E-718.4805161
Produced by FastQC (version 0.11.5)