FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084072
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37420.3451800249429927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6450.034.5356942
GTGGTAT6350.034.22921
GGTATCA16650.029.4136661
GTATCAA34700.028.1476551
TATCAAC38350.025.3135012
TCAACGC39450.024.1108064
CAACGCA39700.024.0985765
ATCAACG39900.023.9110833
AACGCAG40200.023.6642516
ACGCAGA41000.023.2025097
AGAGTAC41500.022.66109311
CGCAGAG42450.022.4757658
CAGAGTA42400.022.30978810
GCAGAGT44650.021.3703129
TGCCGAC5550.020.81322155
AGTACGG46450.019.9520313
GTATCCG700.004961914419.6523881
TCGCGAG700.00497650619.64241230
GAGTACG47650.019.62270512
CGCGTCG750.00741456218.33207127
Produced by FastQC (version 0.11.5)