FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986341
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43460.4406184068187371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6950.030.8713651
GTATCAA33400.030.4720251
TGGTATC7150.029.6065942
GGTATCA14900.029.3532961
CGGTCGA400.00787621427.49741229
TATCAAC37600.026.980022
CGATCGT651.0887551E-425.38480247
ATCAACG40600.024.8484753
TCAACGC40650.024.7490334
CAACGCA41150.024.5151145
CGCAGAG42050.024.250668
AGAGTAC41700.024.1344211
CCGATCG801.6078382E-524.06267746
CAGAGTA42000.024.0214110
AACGCAG42700.023.7527666
ACGCAGA43100.023.5323227
GCAGAGT45000.022.6643439
ACGGGTA1406.4555934E-921.60949316
AGTACGG47200.020.9185913
GAGTACG48800.020.34132412
Produced by FastQC (version 0.11.5)