FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149004
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68100.5926872317241715No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6900.031.5005191
TGGTATC7350.029.9358122
GTATCAA35200.029.154521
TATCAAC38500.026.789152
GGTATCA16750.026.6096941
CAACGCA40500.025.4529295
TACGCGA651.08898035E-425.38455429
TCAACGC40100.025.3640674
ATCAACG40600.025.1215953
AACGCAG41600.024.7170436
ACGCAGA42800.024.024047
AGAGTAC42050.023.87033511
CGCAGAG43100.023.7292448
CAGAGTA42550.023.65137910
GCAGAGT45850.022.307959
AGTACGG47400.021.2350513
GAGTACG49350.020.2836412
AATCGTA1501.4299076E-820.16837518
CGAATCG1651.9626896E-920.00169416
CTGCCGA8300.019.88120355
Produced by FastQC (version 0.11.5)