FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723513
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT25990.3592195302641418No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT7320.10117302660767671TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19450.046.116081
GCGTATA409.306492E-442.5098531
TGGTATC5250.041.2867242
GTGGTAT5400.040.9354131
TATCAAC22400.040.034462
TCAACGC23150.038.3676154
CAACGCA23400.038.139325
ATCAACG23650.037.556463
AACGCAG24950.035.7699436
ACGCAGA25600.034.8593147
CGCAGAG25800.034.5890858
GGTATCA10100.032.40851
GCAGAGT29600.030.1485949
CAGAGTA24150.023.84139410-11
GTACTAT1754.3313776E-721.8622111
AGAGTAC23100.021.43006310-11
CCTCGTA1000.003156627821.247589
TAGACCA1656.3711304E-620.6051395
GTATAGG1708.227809E-620.0046351
AGCGAGC1507.14749E-519.8310768
Produced by FastQC (version 0.11.5)