FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854201
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39540.462888711204974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4900.030.8753381
GTATCAA23500.029.7310961
TGGTATC5200.029.6091732
GGTATCA11500.028.4638881
TATCAAC27350.025.0313132
CGGGATT2300.023.91783117
TCAACGC28450.023.8632264
ATCAACG28650.023.6980293
CAACGCA28800.023.6658885
AACGCAG29700.023.1365186
ACGCAGA30050.022.8670437
CTGCCGA6050.022.73182555
CGCAGAG30250.022.6263168
AGAGTAC30950.022.21119111
CAGAGTA31850.021.75240310
AATATCG650.003250735821.1407435
GCAGAGT33100.020.680559
TGCCGAC3600.020.6291355
ACGGGAT7800.020.1001816
GCCGACG1802.6921043E-1019.86508855
Produced by FastQC (version 0.11.5)