FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061415
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24650.041.4023481
GGTATCA13850.037.1506351
TATCAAC28800.035.721592
TCAACGC30150.033.8353274
TGGTATC7050.033.768112
ATCAACG30300.033.5275423
CAACGCA30950.032.9607475
GTGGTAT7200.032.4833561
AACGCAG31700.032.316536
ACGCAGA34150.029.9924147
CGCAGAG34500.029.5649578
GCAGAGT38400.026.6729439
ATTAGAC1308.0180325E-726.157313
GTATTAG3850.022.0902161
CAGAGTA32350.021.93952810-11
AGAGTAC30650.020.17518810-11
GTATAGG2201.8127685E-719.3289381
GAGTACG36550.019.18542512-13
GTACGGG37250.018.59627514-15
AGTACGG33450.018.48594912-13
Produced by FastQC (version 0.11.5)