FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061415
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22800.21480759175251907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.031.617131
GTATCAA24400.030.5587461
TATCAAC27650.026.8534132
CGGGATA858.785464E-725.88468217
ATCAACG29100.025.508143
TCAACGC29000.025.3105094
CAACGCA29600.024.8879875
AACGCAG29900.024.7337026
GTGGTAT5200.023.8103181
TGGTATC5000.023.6498992
ACGCAGA31300.023.6262917
CGCAGAG31250.023.576128
AGAGTAC30300.023.50317811
CAGAGTA30900.023.13469710
GCAGAGT33500.021.8316129
AGTACGG33800.020.91164413
TGCCGAC3950.020.88992355
GAGTACG35900.019.76035312
GTATATA1151.2056218E-519.1402761
GTACGGG38200.018.43097914
Produced by FastQC (version 0.11.5)