FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580173
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10900.18787499590639345No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG150.00577919655.00715637
CCGATCG257.854889E-443.9981446
CGATCGT300.001924422936.66827847
GTGGTAT3650.033.9260751
TGGTATC3700.032.701322
GTATCAA16500.028.351691
GGTATCA8050.027.3468991
TATCAAC17950.025.1242852
GATCGTA550.001225508325.00109748
TACGCGA550.001226124524.99894129
ATCAACG18050.024.9786323
CAACGCA18400.024.8023195
AGAGTAC18300.024.49138811
TCAACGC18700.024.2594974
AACGCAG18950.024.2275376
ACGCAGA19950.023.4285537
CGCAGAG20050.023.1745748
TCACACG952.3135472E-623.15691429
CAGAGTA19800.022.91372110
GAACGTC752.8711048E-421.9990746
Produced by FastQC (version 0.11.5)