FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570789
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.16135559725222454No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT7240.12684196787254134No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.10494245684482358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG200.003224033263.74319
GTGGTAT3850.049.6830061
GTATCAA16150.043.9541631
TGGTATC4400.042.4991232
TATCAAC18100.039.4466972
ATCAACG19000.037.3512233
CAACGCA19050.037.2531855
TCAACGC19100.036.9331744
GGTATCA8550.036.7893141
AACGCAG19900.035.661976
ACGCAGA21600.032.855247
CGCAGAG21600.032.855248
GCAGAGT24300.029.5544159
ACGGGTC501.0566907E-425.4972416-17
CAGAGTA17700.025.08909410-11
TTATAGT900.001711091323.6085574
GTACTAT1000.003150910421.2532861
GAGTACG20800.021.247712-13
AGAGTAC17000.021.247710-11
GTACGGG21000.020.64062314-15
Produced by FastQC (version 0.11.5)