FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570789
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17790.3116738409464794No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA257.874556E-443.975683
TTATATA401.9065334E-434.3564
GTGGTAT3250.033.8630451
GGTATCA9750.028.2192041
GTATCAA17450.027.2772161
ACACGCC651.0914678E-425.3728078
TGGTATC4150.025.1756292
TATCAAC19300.024.9301872
ATCAACG20150.023.8701733
CAACGCA20500.023.4626355
AACGCAG20800.023.3905566
TCAACGC20450.023.3856014
AGAGTAC20150.022.78695111
ACGCAGA21350.022.6592457
TATACTT852.5783822E-522.6345425
TTGTACC1003.6273632E-621.987844
CTAGTAG752.8809565E-421.987843
CGCAGAG21600.021.887968
CAGAGTA21350.021.76183910
GCCTAAC650.0032284221.1644041
Produced by FastQC (version 0.11.5)