FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909749
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35820.3937349752514155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.030.7551
TGGTATC7600.030.7453882
GTATCAA37400.028.6082631
GTGGTAT8300.028.4992771
TATCAAC41250.026.257112
TCGACGC651.08734326E-425.38933837
CGATCGT550.001225421425.00327547
TCAACGC43750.024.56284
ATCAACG44350.024.417753
AGAGTAC41500.024.24818611
CAACGCA44750.024.138075
AACGCAG45650.023.7839076
CAGAGTA42350.023.62906310
CGCAGAG46400.022.9255568
ACGCAGA48250.022.3313857
TGCCGAC4250.022.00530255
GCAGAGT48600.021.7770549
AGTACGG46100.021.23688913
CCGATCG650.003236776521.15661846
GAGTACG47350.020.73091112
Produced by FastQC (version 0.11.5)