FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802474
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.11414700040125912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5050.043.7648122
GTATCAA22750.043.5434651
GGTATCA11600.039.216991
GTGGTAT5550.039.06841
TATCAAC25600.038.849822
TCAACGC27050.036.293664
ATCAACG27650.035.5060963
CAACGCA27750.035.3781435
AACGCAG28350.034.6294026
CGCAGAG30050.033.092568
ACGCAGA29800.032.9423647
GCAGAGT33650.029.2996169
CAGAGTA26850.024.69103410-11
ACTATAC1102.015269E-423.1816653
TATAGGG1903.871901E-822.3696672
TGGACCG1353.1723248E-522.0368925
GAGTACG31100.021.18021412-13
AGAGTAC26200.020.68082610-11
TATACAG1656.3704556E-620.6059255
GGACCGC1050.0041857320.237966
Produced by FastQC (version 0.11.5)