FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802474
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19840.24723542444988872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23050.028.4120221
GGTATCA11000.027.7668341
ATACGAC400.00788437127.4909673
TGGTATC4650.027.1987532
GTGGTAT5100.026.977141
TATCAAC25750.025.3055172
CAACGCA26550.024.5414895
ATCAACG26700.024.4020943
TCAACGC26650.024.344724
AACGCAG27850.023.3959246
ACGCAGA28250.023.0646537
AGAGTAC27600.023.01591311
CGCAGAG28450.022.9025148
CAGAGTA28100.022.79926110
TACCCCG852.5743628E-522.641035
GCAGAGT30350.021.4700879
AGTACGG29700.021.0194713
GAGTACG31100.020.25011412
TCGTTTC2450.020.20625738
GTATAAT1401.5189835E-719.6547741
Produced by FastQC (version 0.11.5)