FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860949
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5550.041.366131
GTATCAA19350.039.988451
TGGTATC5950.038.5717742
TCAACGC22150.034.7274134
CAACGCA22200.034.6491975
TATCAAC22250.034.3823282
ATCAACG22550.034.1114043
GGTATCA11200.033.4047931
AACGCAG23150.033.2273066
ACGCAGA24900.030.8920577
CGCAGAG25400.030.4512588
GCAGAGT30000.025.6404069
CAGAGTA22000.024.14653810-11
AGAGTAC20800.021.14679510-11
GTATTAC1857.38557E-720.6830671
GACTAAG1050.00417715920.245331
ATTACCC1050.004187005620.23713
GAGTACG25750.020.13493212-13
ATTAATC2151.4312354E-719.766473
GTACGGG27000.019.1240614-15
Produced by FastQC (version 0.11.5)