FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860949
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20620.23950315291614255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3250.032.9917372
GTATCAA19300.030.7909641
GTGGTAT4200.027.5122971
GGTATCA8400.026.8572431
TATCAAC22150.026.4380742
CAACGCA22950.025.6288385
TCAACGC23000.025.453624
ATCAACG23300.025.0064773
AACGCAG23700.024.8192376
AGAGTAC23850.024.21058811
ACGCAGA24650.023.8627157
CGCAGAG25000.023.5286398
CAGAGTA25000.023.42413510
TCCGTAT600.002042216522.9029453
GCAGAGT27100.021.709179
AGTACGG27000.021.0841813
GAGTACG28450.020.00610412
GATCGAC2005.2750693E-1119.25077220
TGCCGAC4050.019.0175355
TACGGGG11450.018.97456215
Produced by FastQC (version 0.11.5)