FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517328
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8670.16759193393746327No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.030.4574051
GTGGTAT3550.027.8973771
TACTACG805.1626193E-727.5045932
TATCAAC19250.026.7187482
CAACGCA19900.025.559715
ATCAACG19700.025.5400713
CTACGTT651.08945525E-425.3790384
AACGCAG20050.025.3660376
TCAACGC19900.025.2833884
CGAAAGA550.001224449325.00417738
CGCAGAG20750.024.5126848
GGTATCA8150.024.303241
ACGCAGA20950.024.2786757
AGAGTAC19850.024.24134811
TGGTATC4150.023.8594072
CAGAGTA20650.023.83483910
TGCCGAC2700.022.4111555
GCAGAGT22550.022.312179
ACGGGTC1254.6124114E-821.9994216
AGTACGG22150.021.8504413
Produced by FastQC (version 0.11.5)